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Variable frequency pulsed field capillary gel electrophoresis of nucleic acid restriction fragment mobility and resolution is reported. The frequency of the sine wave superimposed on the dc driving field was randomly varied throughout the duration of the electrophoresis in order to enhance the fragment separations. By frequent changes of frequency, fragments of various sizes experienced optimum conditions for separation sometime during the electrophoresis. The mobilities of phi X 174 HaeIII fragments in 9% T linear polyacrylamide were primarily dependent on power dissipation in the capillary, independent of whether the driving field was dc only or modulated. Resolution of long fragments exhibited a strong modulation frequency dependence. Modulated driving fields provided better resolution of the 603-1353 base pair fragments than dc only fields. Modulation protocols with center frequencies near 100 Hz gave the highest resolution.
M J Navin, and
T L Rapp, and
M D Morris
August 1995,
Electrophoresis,
M J Navin, and
T L Rapp, and
M D Morris
January 2001,
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M J Navin, and
T L Rapp, and
M D Morris
September 1992,
Bollettino chimico farmaceutico,
M J Navin, and
T L Rapp, and
M D Morris
January 1992,
Methods in molecular biology (Clifton, N.J.),
M J Navin, and
T L Rapp, and
M D Morris
January 1994,
Methods in molecular biology (Clifton, N.J.),
M J Navin, and
T L Rapp, and
M D Morris
November 1996,
Analytical chemistry,
M J Navin, and
T L Rapp, and
M D Morris
September 2011,
Analytical and bioanalytical chemistry,
M J Navin, and
T L Rapp, and
M D Morris
January 1989,
Electrophoresis,
M J Navin, and
T L Rapp, and
M D Morris
June 1987,
Proceedings of the National Academy of Sciences of the United States of America,
M J Navin, and
T L Rapp, and
M D Morris
January 1990,
Microbiology and immunology,
