-transparent.png){kind=logo}
The effects of pretreatment with the nitric oxide (NO)-releasing substances 3-morpholino-sydnoninime (SIN-1) and nitroglycerin were investigated on relaxations induced by non-adrenergic non-cholinergic (NANC) nerve stimulation, authentic NO and vasoactive intestinal polypeptide (VIP) in the rat gastric fundus. Short periods of electrical stimulation (0.5-16 Hz, 1 ms, pulse trains of 10 s) induced frequency-dependent transient relaxations, previously shown to be mainly mediated by NO. Both SIN-1 (10-100 microM) and nitroglycerin (0.5 mM) pretreatment significantly reduced these electrically induced responses to a similar extent as the inhibitor of the NO biosynthesis L-nitroarginine (30-300 microM). Prolonged periods of electrical stimulation (16 Hz, 1 ms, pulse trains of 180 s) induced a sustained relaxation, previously shown to be mediated by NO and VIP. L-Nitroarginine (30-300 microM) or pretreatment with SIN-1 (100 microM) or nitroglycerin (0.5 mM) did not affect the amplitude of this relaxation but slowed down its onset. Authentic NO (0.01-10 microM) and VIP (0.01-10 nM) induced respectively transient and sustained concentration-dependent relaxations. SIN-1 or nitroglycerin pretreatment had no effect on the concentration-response curves to NO and VIP. These results indicate that prolonged exposure to NO donors inhibits electrically induced nerve-mediated NANC relaxations without affecting the postjunctional response to NO and VIP. As similar results are obtained with NO biosynthesis inhibitors, our results illustrate a prejunctional inhibitory effect of NO on the NANC nerves of the rat gastric fundus and suggest the presence of an autoregulatory mechanism for the nitrergic innervation.
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
December 1995,
Nihon Kyobu Shikkan Gakkai zasshi,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
December 1997,
Comparative biochemistry and physiology. Part A, Physiology,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
May 1990,
Nature,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
January 1996,
Archives de pediatrie : organe officiel de la Societe francaise de pediatrie,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
January 1991,
Acta physiologica Scandinavica,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
June 1983,
British journal of pharmacology,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
January 1992,
Japanese journal of pharmacology,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
January 1992,
Japanese journal of pharmacology,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
December 1995,
Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi,
J G De Man, and
G E Boeckxstaens, and
B Y De Winter, and
T G Moreels, and
A G Herman, and
P A Pelckmans
April 1995,
Journal of autonomic pharmacology,
