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To evaluate the possible role of catalase in gastric ethanol metabolism in rats, we studied acetaldehyde formation from ethanol by gastric mucosal homogenate under various in vitro conditions. Homogenized rat gastric mucosa produced significant amounts of acetaldehyde in a time and ethanol concentration-dependent manner, even in the absence of added NAD. Both acetaldehyde formation and catalase activity peaked around the physiological pH, whereas alcohol dehydrogenase (ADH) activity was in that pH range low and reached peak values only at a higher pH of 9 to 10. Catalase inhibitors sodium azide (SA) and 3-amino-1,2,4-triazole (3-AT) had little effect on ADH activity but markedly decreased catalase activity and acetaldehyde formation (1 mM of SA to 56 +/- 13% of control, 5 mM of 3-AT to 67 +/- 3% of control; mean +/- SE). 4-Methylpyrazole decreased ADH activity significantly, but did not affect acetaldehyde formation. Heating of the homogenate at 60 degrees C for 5 min decreased ADH activity only slightly, but totally abolished catalase activity and reduced acetaldehyde formation to 39 +/- 3% of control. Addition of a H2O2 generating system (beta-D(+)-glucose + glucose oxidase] increased acetaldehyde formation in a concentration-dependent manner up to 8-fold of the control value. Our results strongly suggest that, in addition to ADH, catalase may play a significant role in gastric ethanol metabolism in rats.
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
June 1992,
The Journal of clinical investigation,
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
January 1956,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
January 1986,
Pharmacology,
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
May 1997,
The Tokai journal of experimental and clinical medicine,
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
May 1980,
European journal of pharmacology,
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
October 1989,
Gastroenterologia Japonica,
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
February 1990,
Canadian journal of physiology and pharmacology,
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
January 1991,
Alcohol and alcoholism (Oxford, Oxfordshire). Supplement,
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
July 1992,
Biochemical pharmacology,
K S Salmela, and
P Kaihovaara, and
M Salaspuro, and
R P Roine
August 2001,
Fundamental & clinical pharmacology,
