-transparent.png){kind=logo}
This study was undertaken to determine the effect of the immunosuppressant cyclosporin A on neurotransmitter release from non-adrenergic, non-cholinergic nerves (tachykininergic nerves) in the rabbit iris sphincter muscle. Cumulative application of cyclosporin A (0.1 to 10 microM) caused a slow onset of contraction in a concentration-dependent manner. Both FK888 (1 microM) and capsaicin (10 microM), a substance P receptor antagonist and a substance P-depleting agent, respectively, inhibited the contractile effect of cyclosporin A, whereas atropine (1 microM) had no effect. Both cyclosporin A and capsaicin (10 microM) stimulated the release of substance P-like immunoreactivity in the iris. Neither the sodium channel blocker tetrodotoxin (1 microM), the N-type voltage-dependent Ca2+ channel blocker omega-conotoxin GVIA (1 microM) nor the P-type channel blocker omega-agatoxin IVA (0.2 microM) affected cyclosporin A (1 microM)-induced contraction. In contrast, the L-type Ca2+ channel blocker nicardipine (10 microM) inhibited this contractile effect. These results suggest that cyclosporin A stimulates substance P-like tachykinin release by activating L-type voltage-dependent Ca2+ channels, resulting in contraction of the rabbit iris sphincter muscle.
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
December 1993,
British journal of pharmacology,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
March 1985,
Naunyn-Schmiedeberg's archives of pharmacology,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
November 1983,
Biochemical pharmacology,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
March 1993,
Zhonghua nei ke za zhi,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
November 1980,
Nature,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
May 1990,
Nature,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
December 1995,
Nihon Kyobu Shikkan Gakkai zasshi,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
May 1994,
British journal of pharmacology,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
January 1992,
Japanese journal of pharmacology,
M Kageyama, and
H Fujita, and
K Nakata, and
E Shirasawa, and
A Kanai
January 1997,
Current opinion in nephrology and hypertension,
