Functional expression of P2Y purinoceptor in Xenopus oocyte injected with brain mRNA. 1991

E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
Laboratorie de Physiologie Cellulaire UFR de Biologie, Université des Sciences et Techniques de Lille-Flandres-Artois, Villeneuve d'Ascq, France.

Xenopus oocytes injected with embryonic guinea-pig brain mRNA expressed functional P2Y purinoceptors. Extracellular ATP stimulated in a dose-dependent manner a delayed Ca(2+)-dependent Cl- current component. Analysis of the interactions of ATP with compounds that affect Ca2+ fluxes through the plasma membrane or Ca2+ release from internal stores indicates that ATP raises [Ca2+]i by a mechanism that involves activation of voltage-dependent Ca2+ channels, which leads to influx of extracellular Ca2+ into the cells, as well as release of Ca2+ from intracellular stores. Since this phenomenon was not found in control oocytes, it is suggested that brain mRNA encoded for a newly synthesized Ca(2+)-release process stimulated by purinoceptor activation. This mechanism could be largely involved in the short-term regulation of intracellular Ca2+ level involved in ATP neuromodulation functions.

UI MeSH Term Description Entries
D007267 Injections Introduction of substances into the body using a needle and syringe. Injectables,Injectable,Injection
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D011983 Receptors, Purinergic Cell surface proteins that bind PURINES with high affinity and trigger intracellular changes which influence the behavior of cells. The best characterized classes of purinergic receptors in mammals are the P1 receptors, which prefer ADENOSINE, and the P2 receptors, which prefer ATP or ADP. Methyladenine Receptors,Purine Receptors,Purinergic Receptor,Purinergic Receptors,Purinoceptors,Purine Receptor,Purinoceptor,Receptors, Methyladenine,Receptors, Purine,Receptor, Purine,Receptor, Purinergic
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002712 Chlorides Inorganic compounds derived from hydrochloric acid that contain the Cl- ion. Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D004594 Electrophysiology The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
January 1990, Biology of the cell,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
September 1987, Brain research,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
January 1984, Nature,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
March 1994, Brain research. Molecular brain research,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
March 1988, Journal of neurochemistry,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
September 1990, Biochemical and biophysical research communications,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
May 1995, Pharmacology & toxicology,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
July 1995, Brain research. Developmental brain research,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
March 1990, FEBS letters,
E Honoré, and F Fournier, and T Collin, and J Nargeot, and P Guilbault
January 1995, Masui. The Japanese journal of anesthesiology,
Copied contents to your clipboard!